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c 6 nbd sm  (Biotium)


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    Structured Review

    Biotium c 6 nbd sm
    C 6 Nbd Sm, supplied by Biotium, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c 6 nbd sm/product/Biotium
    Average 91 stars, based on 3 article reviews
    c 6 nbd sm - by Bioz Stars, 2026-02
    91/100 stars

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    Thermo Fisher c 6 -nbd-sm {6-[ n -(7-nitro-2,1,3benzoxadiazol-4-yl)amino]hexanoylsphingosylphosphocholine}
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    Mice of the indicated genotypes were placed on either a standard or modified diet for 10 weeks. S-SMase activity was measured using <t>C</t> <t>6</t> <t>-NBD-SM</t> as a substrate. ( A ) S-SMase activity in C57Bl6 mice measured by using 0.4 nmol of NBD-SM per sample. Results are shown as means±S.D. ( n =3) for each mouse. ( B–D ) Michaelis–Menten kinetics of the S-SMase activity in C57Bl6 and ldlr −/− mice on either standard or modified diet. The assays are carried out using serum (4–6 animals per assay) and the indicated substrate concentrations. Mean values of triplicates ±S.D. are shown. * P <0.05 and ** P <0.01 according to a Student's t test. SM/NBD-SM ratio in ( D ) was calculated by dividing the measured endogenous LDL-SM concentration (presented in ) and the amount of NBD-SM in each assay.
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    Mice of the indicated genotypes were placed on either a standard or modified diet for 10 weeks. S-SMase activity was measured using <t>C</t> <t>6</t> <t>-NBD-SM</t> as a substrate. ( A ) S-SMase activity in C57Bl6 mice measured by using 0.4 nmol of NBD-SM per sample. Results are shown as means±S.D. ( n =3) for each mouse. ( B–D ) Michaelis–Menten kinetics of the S-SMase activity in C57Bl6 and ldlr −/− mice on either standard or modified diet. The assays are carried out using serum (4–6 animals per assay) and the indicated substrate concentrations. Mean values of triplicates ±S.D. are shown. * P <0.05 and ** P <0.01 according to a Student's t test. SM/NBD-SM ratio in ( D ) was calculated by dividing the measured endogenous LDL-SM concentration (presented in ) and the amount of NBD-SM in each assay.
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    Image Search Results


    Mice of the indicated genotypes were placed on either a standard or modified diet for 10 weeks. S-SMase activity was measured using C 6 -NBD-SM as a substrate. ( A ) S-SMase activity in C57Bl6 mice measured by using 0.4 nmol of NBD-SM per sample. Results are shown as means±S.D. ( n =3) for each mouse. ( B–D ) Michaelis–Menten kinetics of the S-SMase activity in C57Bl6 and ldlr −/− mice on either standard or modified diet. The assays are carried out using serum (4–6 animals per assay) and the indicated substrate concentrations. Mean values of triplicates ±S.D. are shown. * P <0.05 and ** P <0.01 according to a Student's t test. SM/NBD-SM ratio in ( D ) was calculated by dividing the measured endogenous LDL-SM concentration (presented in ) and the amount of NBD-SM in each assay.

    Journal: Bioscience Reports

    Article Title: Characterization of secretory sphingomyelinase activity, lipoprotein sphingolipid content and LDL aggregation in ldlr −/− mice fed on a high-fat diet

    doi: 10.1042/BSR20120036

    Figure Lengend Snippet: Mice of the indicated genotypes were placed on either a standard or modified diet for 10 weeks. S-SMase activity was measured using C 6 -NBD-SM as a substrate. ( A ) S-SMase activity in C57Bl6 mice measured by using 0.4 nmol of NBD-SM per sample. Results are shown as means±S.D. ( n =3) for each mouse. ( B–D ) Michaelis–Menten kinetics of the S-SMase activity in C57Bl6 and ldlr −/− mice on either standard or modified diet. The assays are carried out using serum (4–6 animals per assay) and the indicated substrate concentrations. Mean values of triplicates ±S.D. are shown. * P <0.05 and ** P <0.01 according to a Student's t test. SM/NBD-SM ratio in ( D ) was calculated by dividing the measured endogenous LDL-SM concentration (presented in ) and the amount of NBD-SM in each assay.

    Article Snippet: C 6 -NBD-Cer {6-[ N -(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoylceramide} and C 6 -NBD-SM {6-[ N -(7-nitro-2,1,3benzoxadiazol-4-yl)amino]hexanoylsphingosylphosphocholine} were from Molecular Probes, C 17:0 ceramide ( N -heptadecanoyl- D - erythro -sphingosine), C 17:0 SM ( N -heptadecanoyl- D - erythro -sphingosylphosphocholine), N -acetyl-C 20 -sphinganine and bovine brain ceramide were purchased from Avanti Polar Lipids. bSMase was from Sigma–Aldrich.

    Techniques: Modification, Activity Assay, Concentration Assay